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The commercially cultivated edible oyster mushrooms Pleurotus sajor-caju and P. pulmonarius are two separate species, similar in morphology but reproductively isolated

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Abstract

Two closely related commercially cultivated oyster mushroom species, Pleurotus pulmonarius and P. sajor-caju have been differentiated by traditional mating experiments as well as analysis of the variable ITS and IGS sequences of the ribosomal gene cluster. Molecular analysis of the variable ITS and IGS regions has allowed neither reliable differentiation between the morphologically similar species P. pulmonarius and P. sajor-caju nor confirmation of species identity of the P. sajor-caju strains CS-32, H-1, and H-2. Analysis of the sexual (mating) compatibility between haploid tester strains of these two species in monokaryon-monokaryon mating experiments has demonstrated complete reproductive isolation between P. pulmonarius and P. sajor-caju, thereby confirming that these are separate species.

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References

  1. Bonatti, M., Karnopp, P., Soares, H.M., and Furlan, S.A., Evaluation of Pleurotus ostreatus and Pleurotus sajorcaju Nutritional Characteristics When Cultivated in Different Lignocellulosic Wastes, Food Chem., 2004, vol. 88, no. 3, pp. 425–428.

    Article  CAS  Google Scholar 

  2. Zhuang, C., Li, W., and Chen, S., Antitumor Protein-Containing Polysaccharides from a Chinese Mushroom Jengweigu or Houbitake, Pleurotus sajor-caju (Fr.) Sing, Boisci. Biotechnol. Biochem., 1993, no. 57, pp. 901–906.

  3. Ngai, P.H. and Ng, T.B., A Ribonuclease with Antimicrobial, Antimitogenic and Antiproliferative Activities from the Edible Mushroom Pleurotus sajor-caju, Peptides, 2004, vol. 25, no. 1, pp. 11–17.

    Article  PubMed  CAS  Google Scholar 

  4. Lakshmi, B., Tilak, J.C., Adhikari, S., et al., Inhibition of Lipid Peroxidation Induced by γ-Radiation and AAPH in Rat Liver and Brain Mitochondria by Mushrooms, Curr. Sci., 2005, vol. 88, no. 3, pp. 484–488.

    CAS  Google Scholar 

  5. Kashangura, C., Hallsworth, J., and Mswaka, A., Phenotypic Diversity amongst Strains of Pleurotus sajorcaju: Implications for Cultivation in Arid Environments, Mycol. Res., 2006, vol. 110, no. 3, pp. 312–317.

    Article  PubMed  CAS  Google Scholar 

  6. Chiua, S.W., Chana, Y.H., Lawa, S.C., et al., Cadmium and Manganese in Contrast to Calcium Reduce Yield and Nutritional Values of the Edible Mushroom Pleurotus pulmonarius, Mecoplogist, 1998, vol. 12, pp. 56–64.

    Google Scholar 

  7. Idowu, O., Evaluation of Different Substrates and Combinations on the Growth of Pleurotus pulmonarius (Fries) Quelet (P. sajor-caju), Nigerian J. Hortic. Sci., 2003, vol. 8, pp. 112–221.

    Google Scholar 

  8. Corner, E.J.H., The Agaric Genera Lentinus, Panus, and Pleurotus with Particular Reference to Malaysian Species, Nova Hedwigia, 1981, vol. 69,suppl., pp. 1–169.

    Google Scholar 

  9. Kurtzman, R.H. and Zadrazil, F., Physiological and Taxonomic Considerations for Cultivation of Pleurotus Mushrooms, Tropical Mushrooms: Biological Nature and Cultivation Methods, Chang, S.T. and Quimio, T.H., Eds., Hong Kong: Chin. Univ. Press, 1982, pp. 299–348.

    Google Scholar 

  10. Pegler, D.N., The Genus Lentinus, Kew Bull. Additional Ser., 1983, no. 10, pp. 1–281.

  11. Hilber, O., Valid, Invalid and Confusing Taxa of the Genus Pleurotus, Mushroom Sci., 1989, vol. 12, pp. 241–248.

    Google Scholar 

  12. Altukhov, Yu.P., Species and Speciation, Sorosovsky Obrazovatelny Zh., 1997, no. 4, pp. 1–10.

  13. Eger, G., Biology and Breeding of Pleurotus, in The Biology of Cultivation of Edible Mushrooms, New York: Academic, 1978.

    Google Scholar 

  14. Shnyreva, A.V., Druzhinina, I.S., and D’yakov, Yu.T., Genetic Structure of the Pleurotus ostreatus sensu lato Complex in Moscow Region, Russ. J. Genet., 1998, vol. 34, no. 12, pp. 1371–1378.

    CAS  Google Scholar 

  15. Vilgalys, R. and Sun, B.L., Assessment of Species Distributions in Pleurotus Based on Trapping of Airborne Basidiospores, Mycologia, 1994, vol. 86, no. 2, pp. 270–274.

    Article  Google Scholar 

  16. Zervakis, G. and Balis, C., A Pluralistic Approach in the Study of Pleurotus Species with Emphasis on Compatibility and Physiology of the European Morphotaxa, Mycol. Res., 1996, vol. 100, no. 6, pp. 717–731.

    Article  Google Scholar 

  17. Lee, S.B., Milgroom, M.G., and Taylor, J.W., A Rapid, High Yield Mini-Prep Method for Isolation of Total Genomic DNA from Fungi, Fungal Genet. Biol., 1988, vol. 35, pp. 23–24.

    Google Scholar 

  18. Sambrook, J., Fritsch, E.F., and Maniatis, T.A., Molecular Cloning: A Laboratory Manual, New York: Cold Spring Harbor Lab., 1989.

    Google Scholar 

  19. Nilsson, R.H., Kristiansson, E., Ryberg, M., et al., Intraspecific ITS Variability in the Kingdom Fungi as Expressed in the Internal Sequence Databases and Its Implications for Molecular Species Identification, Evol. Bioinform., 2008, vol. 4, pp. 193–201.

    Google Scholar 

  20. Hall, T.A., BioEdit: A User-Friendly Biological Sequence Alignment Editor and Analysis Program for Windows 95/98/NT, Nucleic Acids Symp. Ser., 1999, vol. 41, pp. 95–98.

    CAS  Google Scholar 

  21. Tamura, K., Peterson, D., Peterson, N., et al., MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods, Mol. Biol. Evol., 2011, vol. 28, pp. 2731–2739, www.kumarlab.net/publications.

    Article  PubMed  CAS  Google Scholar 

  22. Stamets, P., Growing Gourmet and Medicinal Mushrooms, Berkley: Ten Speed Press, 1993.

    Google Scholar 

  23. Garibova, L.V. and Sidorova, I.I., Griby (Mashrooms), in Entsiklopediya prirody Rossii (Encyclopedia of the Nature of Russia), Moscow, 1997.

  24. Shnyreva, A.V., Genesystematics and the Species Problem in Fungi: Approaches and Solutions, Mikol. Fitopatol., 2011, vol. 45, no. 3, pp. 209–220.

    Google Scholar 

  25. Shnyreva, A.V. and Shtaer, O.V., Differentiation of Closely Related Oyster Fungi Pleurotus pulmonarius and P. ostreatus by Mating and Molecular Markers, Russ. J. Genet., 2006, vol. 42, no. 5, pp. 667–674.

    Article  CAS  Google Scholar 

  26. D’yakov, Yu.T., Reproductive Systems and Their Evolution in Fungi, Mikol. Fitopatol., 1999, vol. 33, no. 3, pp. 137–149.

    Google Scholar 

  27. Mayr, E., Principles of Systematic Zoology, New York: McGraw-Hill, 1969.

    Google Scholar 

  28. Anderson, J.B. and Kohn, L.M., Genotyping, Gene Genealogies and Genomics Bring Fungal Population Genetics above Ground, Trends Ecol. Evol., 1998, vol. 13, no. 11, pp. 444–449.

    Article  PubMed  CAS  Google Scholar 

  29. Shnyreva, A.V., Population Genetics of Mushrooms, Mikologiya segodnya (Mycology Today), D’yakov, Yu.T. and Sergeev, Yu.V, Eds., Moscow: National Academy of Mycology, 2007, pp. 195–225.

    Google Scholar 

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Authors and Affiliations

  1. Department of Mycology and Algology, Lomonosov Moscow State University, Moscow, 119991, Russia

    A. A. Shnyreva, A. B. Sivolapova & A. V. Shnyreva

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  1. A. A. Shnyreva
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  2. A. B. Sivolapova
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  3. A. V. Shnyreva
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Correspondence to A. A. Shnyreva.

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Original Russian Text © A.A. Shnyreva, A.B. Sivolapova, A.V. Shnyreva, 2012, published in Genetika, 2012, Vol. 48, No. 11, pp. 1260–1270.

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Shnyreva, A.A., Sivolapova, A.B. & Shnyreva, A.V. The commercially cultivated edible oyster mushrooms Pleurotus sajor-caju and P. pulmonarius are two separate species, similar in morphology but reproductively isolated. Russ J Genet 48, 1080–1088 (2012). https://doi.org/10.1134/S1022795412110105

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  • DOI: https://doi.org/10.1134/S1022795412110105

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